human ec cell lines  (Procell Inc)

Journal: Scientific Reports

Article Title: Integrative network toxicology and experimental evidence reveal mechanisms underlying diethyl phthalate-induced initiation and progression of endometrial cancer

doi: 10.1038/s41598-026-39325-6

Figure Lengend Snippet: DEP inhibited proliferation and induced ROS accumulation in EC cells. ( A , B ) CCK-8 assay showing the effect of different concentrations of DEP on proliferation of HEC-1-A ( A ) and Ishikawa ( B ) cells at 0, 24, and 48 h (n = 3). ( C , D ) Representative EdU staining images of HEC-1-A ( C ) and Ishikawa ( D ) cells treated with DEP at the indicated concentrations (DMSO, DEP at 10 nM, 1 μM, and 10 μM). Nuclei were counterstained with Hoechst 33,342 (blue), and EdU-positive cells are shown in red. Scale bar = 100 μm. ( E ) Quantification of EdU-positive cells in HEC-1-A and Ishikawa cell lines (n = 3). ( F ) Representative fluorescence images of ROS staining in HEC-1-A and Ishikawa cells following DEP treatment. White arrows indicate cells with increased ROS levels, and blue arrows indicate cells with lower ROS levels (n = 3). Scale bar = 20 μm. Data are presented as mean ± SD; statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. (* P < 0.05, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human EC cell lines HEC-1-A and Ishikawa were obtained from GeneChem Gene Technology Co., Ltd. (Shanghai, China).

Techniques: CCK-8 Assay, Staining, Fluorescence

Journal: Scientific Reports

Article Title: Integrative network toxicology and experimental evidence reveal mechanisms underlying diethyl phthalate-induced initiation and progression of endometrial cancer

doi: 10.1038/s41598-026-39325-6

Figure Lengend Snippet: DEP induced cell cycle arrest and modulated the PI3K/AKT signaling pathway in EC cells. ( A ) Flow cytometry analysis of cell cycle distribution in Ishikawa and HEC-1-A cells treated with DEP at the indicated concentrations (DMSO, DEP at 10 nM, 1 μM, and 10 μM). ( B ) Quantification of the proportion of cells in G0/G1, S, and G2/M phases (n = 3). ( C ) WB analysis of PI3K, P-PI3K, AKT, P-AKT, ERK, P-ERK, CDK4, Cyclin D1, GSK3β, and P-GSK3β expression in Ishikawa and HEC-1-A cells after DEP treatment. ( D ) Densitometric quantification of protein expression levels normalized to GAPDH or β-actin (n = 3). Data are presented as mean ± SD; statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. (ns = not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human EC cell lines HEC-1-A and Ishikawa were obtained from GeneChem Gene Technology Co., Ltd. (Shanghai, China).

Techniques: Flow Cytometry, Expressing